Selective N-terminal acylation of peptides and proteins with a Gly-His tag sequence
نویسندگان
چکیده
منابع مشابه
Selective N-terminal functionalization of native peptides and proteins.
We report an efficient, highly selective modification on the N-terminal amines of peptides and proteins using aldehyde derivatives via reductive alkylation. After modification of a library of unprotected peptides XYSKEASAL (X varies over 20 natural amino acids) by benzaldehyde at room temperature, pH 6.1 resulted in excellent N-terminal selectivity (α-amino/ε-amino: >99 : 1) and high reaction c...
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N-hydroxysuccinimide (NHS) esters are derivatizing agents that target primary amine groups. However, even a small molar excess of NHS may lead to acylation of hydroxyl-containing amino acids as a side reaction. We report a straightforward method for the selective removal of ester-linked acyl groups after NHS ester-mediated acylation of peptides and proteins. It is based on incubation in a boili...
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The authors apologise for this error. Figure 4. His-tag effect on uneven distribution and activity of chloramphenicol acetyltransferase (CAT), neopullulanase (BCN) and esterase 1767 (A) Distinct uneven distribuiton of proteins with N-terminal His-tag were observed by immunofluorescence staining using anti-His-tag antibody. As a negative conrol, unlocalized patterns of mBFP (without His-tag) usi...
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ژورنال
عنوان ژورنال: Nature Communications
سال: 2018
ISSN: 2041-1723
DOI: 10.1038/s41467-018-05695-3